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KMID : 0364820090450040397
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Korean Journal of Microbiology
2009 Volume.45 No. 4 p.397 ~ p.403
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Identification and Antioxidant Activity of Marine Actinomycetes Streptomyces sp. ACT-1
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Kim Man-Chul
Kim Ju-Sang
Kim Yun-Beom
Harikrishnan Ramasamy
Han Yong-Jae
Heo Moon-Soo
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Abstract
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For the research of the natural antioxidant from marine sources, an antioxidant-producing marine actinomycetes was isolated from sea water in Jeju coastal area. The strain was identified based on 16S rDNA sequencing, the morphology by a method of scanning electron microscopy, physiological and biochemical characteristics and cellular fatty acid analysis. The isolated strain ACT-1 cell size was 0.5~1.0 μm and gram positive, aerobic, nonmotile, substrate mycelium are red and gray aerial mycelium. 16S rRNA sequence analysis showed that were Gram-positive bacteria grouped on Streptomyces genus. Results of cellular fatty acid analysis showed that major cellular fatty acids were C15:0 anteiso (39.33%), C16:1 cis 9 (11.96%), C16:0 (13.08%) and C17:0 anteiso (10.99%). Finally, strain was identified Streptomyces sp. ACT-1. The antioxidant activity of methanol extract from Streptomyces sp. ACT-1 was evaluated by measuring DPPH, hydroxyl, and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. DPPH radical scavenging activity of SBME-1 (Streptomyces broth methanol extract) was 67% at 1,000 μg/ml. Hydroxyl radical scavenging activity of SBME-1 was 84% at 500 μg/ml. Alkyl radical scavenging activity of SBME-1 was 71% at 1,000 μg/ml.
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KEYWORD
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16S rRNA, antioxidant, Actinomycetes, Streptomyces sp. ACT-1
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